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pETBlue-1

PLASMID

The pETBlue vectors are designed to identify recombinants by traditional blue/white screening while also allowing T7lac promoter based expression of target genes. Screening is independent of expression because the T7lac expression promoter is in an opposed orientation relative to the E. coli promoter that mediates blue/white screening. The EcoR V cloning site is appropriately spaced down- stream of an E. coli ribosome binding site.

Institution
JBEI
ICE ID
JPUB_004939
Creator
Gregory Linshiz
TG Entity Type
DNA-Sequence
TeselaGen ID
6dded9a5-1de9-46d8-895a-a966d0523808
Tag
Linshiz et al. 2016; EMPTY-SEQUENCE

How to Access This Part

  1. Visit the TeselaGen Community portal.
  2. Sign in or create a free account.
  3. Open the corresponding entity library (e.g., DNA Sequences), click on "Import from External DB", select the "ICE Import.." option, and enter the corresponding name: pETBlue-1.
  4. Select the entities, and click Import. A copy of the entities will be copied on your account.
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