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pCM62-Prha-T7SL-RFP

PLASMID

The pCM62 backbone contains the modified, minimal replication region from the broad host range (low copy) RK2 (IncP) replicon. pCM62-Prha-T7SL-RFP contains the rhamnose promoter from pREDI in front of RFP as well a mRNA leader sequence from T7 phage that creates a 5' stem-loop stabilizing the mRNA transcript and increasing expression.

Institution
JBEI
ICE ID
JPUB_010104
Creator
Jonathan Vroom
TG Entity Type
DNA-Sequence
TeselaGen ID
c985576a-d6a3-403b-be3e-ba2b5e3a5b6b
Tag
Goyal et al. 2018

How to Access This Part

  1. Visit the TeselaGen Community portal.
  2. Sign in or create a free account.
  3. Open the corresponding entity library (e.g., DNA Sequences), click on "Import from External DB", select the "ICE Import.." option, and enter the corresponding name: pCM62-Prha-T7SL-RFP.
  4. Select the entities, and click Import. A copy of the entities will be copied on your account.
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