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pTE433-beta

PLASMID

Empty vector plasmid pTE433-beta used to assemble targeted gRNAs for DSB activity at user-defined sequences. Cut the plasmid with PmeI-AgeI or amplify with oligos TEAM-1212/1213. After purification assemble with desired gRNA for recombineering.

Institution
JBEI
ICE ID
JPUB_022570
Creator
Thomas Eng
TG Entity Type
DNA-Sequence
TeselaGen ID
9a64581e-39ee-44f1-b39c-9c8cb896a9a4
Tag
Eng-Banerjee et al 2023, pca/indg PSP

How to Access This Part

  1. Visit the TeselaGen Community portal.
  2. Sign in or create a free account.
  3. Open the corresponding entity library (e.g., DNA Sequences), click on "Import from External DB", select the "ICE Import.." option, and enter the corresponding name: pTE433-beta.
  4. Select the entities, and click Import. A copy of the entities will be copied on your account.
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