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HYD (Kan:P15A)
PLASMIDEncodes C. necator hydrogenase heterotetramer (HoxFUYH) and maturation proteins (HoxWI and HypA2B2C2DEFX), as described in Bertrand et al. 2024a [Manuscript in preparation] and Bertrand et al. 2024b [Manuscript in preparation]. Codon optimized for E. coli and IPTG-inducible. Performs the following reaction when expressed in E. coli: H2 + NAD = H + NADH Additional customization is enabled by KpnI-NotI cloning site. Plasmid is contained within Stellar E. coli (Takara) cloning strain. Plasmid miniprep and transformation into end-user’s desired strain of bacteria will be required to achieve the described application.
- Institution
- JBEI
- ICE ID
- JPUB_026716
- Creator
- Robert Bertrand
- TG Entity Type
- DNA-Sequence
- TeselaGen ID
- dc284be7-7754-4a92-8d47-461dfc4c3081
- Tag
- Bertrand et. al. 2026
How to Access This Part
- Visit the TeselaGen Community portal.
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- Open the corresponding entity library (e.g., DNA Sequences), click on "Import from External DB", select the "ICE Import.." option, and enter the corresponding name:
HYD (Kan:P15A). - Select the entities, and click Import. A copy of the entities will be copied on your account.