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JBEI-258701

STRAIN

BW25113 (with DE3 complement enabling use of T7 promoters). . . First plasmid encodes acetoacetyl-CoA thiolase, HMG-CoA synthase, and HMG-CoA reductase, to enable the NADH-dependent production of mevalonate. Plasmid contains low-copy SC101 origin and was derived from the P15A variant originally created by Kang A, Mendez-Perez D, Goh EB, Baidoo EEK, Benites VT, Beller HR, Keasling JD, Adams PD, Mukhopadhyay A, Lee TS. 2019. Optimization of the IPP-bypass mevalonate pathway and fed-batch fermentation for the production of isoprenol in Escherichia coli. Metab. Eng. 56: 85-96. . . Second plasmid encodes the C. necator hydrogenase plasmid “HYD (Spec:P15A)”, as described in Bertrand et al. 2024b [Title TBD] Manuscript in preparation. . . Strain is robustly functional in my hands, and performs the following reactions:. H2 + NAD+ = H+ + NADH. 3Acetate + 2NADH = Mevalonate + CO2. . For best practice, please request plates.

Institution
JBEI
ICE ID
JPUB_026719
Creator
Robert Bertrand
TG Entity Type
Microbrial-Strain
TeselaGen ID
c5c59c4d-b977-47e7-976b-5c43bdafe24b
Tag
Bertrand et. al. 2026

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