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JBEI-258702

STRAIN

BW25113 (with DE3 complement enabling use of T7 promoters). . . First plasmid encodes flaviolin-producing type III PKS, as reported in Incha MR, Thompson MG, Blake-Hedges JM, Liu Y, Pearson AN, Schmidt M, Gin JW, Petzold CJ, Deutschbauer AM, Keasling JD. 2020. Leveraging host metabolism for bisdemethoxycurcumin production in Pseudomonas putida. Metab. Eng. Comm. 10: e00119. . . The second plasmid encodes C. necator hydrogenase “HYD (Kan:P15A)”, as reported in Bertrand et al. 2024a [Title TBD] Manuscript in preparation. . . Strain is robustly functional in my hands, and performs the following reactions:. . 5 malonyl-CoA + O2 = Flaviolin. H2 + NAD+ = H+ + NADH. . For best practices, please request plates.

Institution
JBEI
ICE ID
JPUB_026727
Creator
Robert Bertrand
TG Entity Type
Microbrial-Strain
TeselaGen ID
06f6441d-99f2-437a-96b6-1a28b663fbfc
Tag
Bertrand et. al. 2026

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  3. Open the corresponding entity library (e.g., DNA Sequences), click on "Import from External DB", select the "ICE Import.." option, and enter the corresponding name: JBEI-258702.
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