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JBEI

Browse the public JBEI registry of synthetic biological parts hosted by TeselaGen.

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Registry Entries(12,525)

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ICE IDNameTypeDescription
JPUB_005321pTrc55KADS-MBIS1PLASMID55000 activity RBS in front of ADS and MBIS1 operon in pTrc99a backbone
JPUB_005320pTrc32KADS-MBIS1PLASMID32000 activity RBS in front of ADS and MBIS1 operon in pTrc99a backbone
JPUB_005319pTrc15KADS-MBIS1PLASMID15000 activity RBS in front of ADS and MBIS1 operon in pTrc99a backbone
JPUB_005318pTrc10KADS-MBIS1PLASMID10000 activity RBS in front of ADS and MBIS1 operon in pTrc99a backbone
JPUB_005317pTrc5KADS-MBIS1PLASMID5000 activity RBS in front of ADS and MBIS1 operon in pTrc99a backbone
JPUB_005316pTrcADS-MBIS8PLASMIDADS gene and MBIS8 opron in pTrc99a backbone
JPUB_005315pTrcADS-MBIS7PLASMIDADS gene and MBIS7 opron in pTrc99a backbone
JPUB_005314pTrcADS-MBIS5PLASMIDADS gene and MBIS5 opron in pTrc99a backbone
JPUB_005313pTrcADS-MBIS4PLASMIDADS gene and MBIS4 opron in pTrc99a backbone
JPUB_005312pTrcADS-MBIS3PLASMIDADS gene and MBIS3 opron in pTrc99a backbone
JPUB_005311pTrcADS-MBIS2PLASMIDADS gene and MBIS2 opron in pTrc99a backbone
JPUB_005310pTrcADS-MBIS1PLASMIDADS gene and MBIS1 opron(medium activity RBS) in pTrc99a
JPUB_004849pBbA5c-MevT-MBISPLASMIDfull mevalonate pathway in one biobrick plasmid
JPUB_005575pBbA5c-MevTPLASMIDpMevT in biobrick format
JPUB_005573pBbA5c-AtoB-HMGSPLASMIDAtoB and HMGS from mevalonate pathway in biobrick format
JPUB_004707pKS1PLASMIDMedium chain acyl-acp thioesterase native to e.coli, but lacking the periplasmic targeting region
JPUB_010097pKD46PLASMIDPlasmid used for the generation of knockout insertions using PCR products by the lambda red recombination pathway.
JPUB_000270pSKIPLASMIDA plasmid PCR template for generating linear DNA fragments containing the KanR and sacB genes, as well as an I-SceI recognition site, to be flanked by E. coli homologous sequences to direct the chromosomal recombination in coordination with the lamda red and I-SceI systems carried on the pREDI plasmid. Note, this is not the original pSKI published, but was derived from pSCI. However, it essentially has the same functionality of the original pSKI.
JPUB_010095pREDIPLASMIDA plasmid bearing the lambda-red recombinase system under the control of arabinose, and the I-SceI endonuclease under the control of rhamnose. The origin is temperature-senstive, allowing for temperature-curing of the plasmid.
JPUB_010093pMCS-4PLASMIDA plasmid bearing the Gentamycin resistance gene aacC1. Used for integrating DNA into the chromosome of Caulobacter crescentus, but replicates in E. coli.
JPUB_008728pBbE5c-YFPPLASMIDBiobricked YFP in lacUV5/colE1 plasmid
JPUB_005138pET-52b+_PMK-HisPLASMIDPhosphomevalonate Kinase (PMK) cut into pET-52b(+) using NcoI & SacI. PMK was amplified for cloning using a primer that created an NcoI overhang and then inserted using BsaI & SacI.
JPUB_007574pLAC33PLASMID
JPUB_007576pLAC33SOE4PLASMIDpSOE4
JPUB_026534pUC19PLASMIDblue/white selection through a-complementation
JPUB_005551pADSmut1PLASMID
JPUB_006315pLac33MevT(C159A)PLASMID
JPUB_012020pMMB-PHAPLASMID
JPUB_004786pADSPLASMIDpADS contains the codon-optimized amorphadiene synthase gene from Artemisia annua
JPUB_006313pMevBPLASMIDMevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse. Comments: Doesn't contain lacI
JPUB_006505pDsRed._T3-S4TPLASMID
JPUB_007700pRS413PLASMIDApplications: YC-type (centromeric) shuttle vector
JPUB_007701pRS416PLASMIDApplications: YC-type (centromeric) shuttle vector
JPUB_026533pUC19PLASMID
JPUB_004784pMBIPLASMIDMevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse, IPP isomerase. Comments: Doesn't contain lacI/ ERG1, ERG8, MVD1: S. cerevisiae/ idi: E. Coli
JPUB_005549pMBISPLASMIDMevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse, IPP isomerase, FPP synthase. Comments: Doesn't contain lacI/ ERG1, ERG8, MVD1: S. cerevisiae/ idi: E. Coli
JPUB_005547pMevTPLASMIDCulture on Cm and 0.1% Glucose! This plasmid does not contain LacI, therefore the operon is constitutively expressed unless the strain is grown with glucose. We have noticed plasmid instability due to the high expression of this operon. pMevT is also known as pLac33MevT in lab books and notes.
JPUB_010091pAH143PLASMID
JPUB_026815Arabidopsis_pSa_K155N_GFPSTRAINGV3101 harboring a constitutive GFP construct with kanamycin selection for A. thaliana. Uses a pSa_K155N backbone.
JPUB_026814Arabidopsis_pSa_V152F_GFPSTRAINGV3101 harboring a constitutive GFP construct with kanamycin selection for A. thaliana. Uses a pSa_V152F backbone.
JPUB_026813Arabidopsis_pSa_WT_GFPSTRAINGV3101 harboring a constitutive GFP construct with kanamycin selection for A. thaliana. Uses a pSa_WT backbone.
JPUB_026812Arabidopsis_pVS1_K310Q_GFPSTRAINGV3101 harboring a constitutive GFP construct with kanamycin selection for A. thaliana. Uses a pVS1_K310Q backbone.
JPUB_026811Arabidopsis_pVS1_R106H_GFPSTRAINGV3101 harboring a constitutive GFP construct with kanamycin selection for A. thaliana. Uses a pVS1_R106H backbone.
JPUB_026810Arabidopsis_pVS1_WT_GFPSTRAINGV3101 harboring a constitutive GFP construct with kanamycin selection for A. thaliana. Uses a pVS1_WT backbone.
JPUB_026809Rhodo_pSa_K155N_GFPSTRAINEHA105 harboring a constitutive GFP construct with nurseothrycin selection for R. toruloides transformation. Uses a pSa_K155N backbone
JPUB_026808Rhodo_pSa_V152F_GFPSTRAINEHA105 harboring a constitutive GFP construct with nurseothrycin selection for R. toruloides transformation. Uses a pSa_V152F backbone
JPUB_026807Rhodo_pSa_WT_GFPSTRAINEHA105 harboring a constitutive GFP construct with nurseothrycin selection for R. toruloides transformation. Uses a pSa_WT backbone
JPUB_026806Rhodo_pVS1_K310Q_GFPSTRAINEHA105 harboring a constitutive GFP construct with nurseothrycin selection for R. toruloides transformation. Uses a pVS1_K310Q backbone
JPUB_026805Rhodo_pVS1_R106H_GFPSTRAINEHA105 harboring a constitutive GFP construct with nurseothrycin selection for R. toruloides transformation. Uses a pVS1_R106H backbone
JPUB_026804Rhodo_pVS1_WT_GFPSTRAINEHA105 harboring a constitutive GFP construct with nurseothrycin selection for R. toruloides transformation. Uses a pVS1_WT backbone

12,525 entries · Page 114 of 251