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JBEI

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Registry Entries(12,525)

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ICE IDNameTypeDescription
JPUB_004777pRSLeu2d-BIS-71PLASMIDThis variant (pRSLeu2d-BIS-71) has the 3' end of the cds recoded (the BIS protein sequence is the same). See reference below for description. Specific bisabolene production is 20% higher than with the wt pRSleu2d-BISopt plasmid when expressed in EPY300.
JPUB_004775pRSLeu2d-BIS-91PLASMIDThis variant (pRSLeu2d-BIS-91) has the 3' end of the cds recoded (the BIS protein sequence is the same). See reference below for description. Specific bisabolene production is 32% higher than with the wt pRSleu2d-BISopt plasmid when expressed in EPY300.
JPUB_004847Cel5A H138R N236DPLASMIDE.coli expression vector (pFIL-B) containing Cel5A from Thermotoga maritima. Gene contains the mutations H138R N236D. Vector has C-terminal TEV cleavage site, Strept tag, 6xHIS tag.
JPUB_004846Cel5A N236DPLASMIDE.coli expression vector (pFIL-B) containing Cel5A from Thermotoga maritima. Gene contains the mutation N236D. Vector has C-terminal TEV cleavage site, Strept tag, 6xHIS tag.
JPUB_004845Cel5A H138RPLASMIDE.coli expression vector (pFIL-B) containing Cel5A from Thermotoga maritima. Gene contains the mutation H138R. Vector has C-terminal TEV cleavage site, Strept tag, 6xHIS tag.
JPUB_004844TIL-67PLASMIDE.coli expression vector (pFIL-B) containing Cel5A from Thermotoga Maritima (gene sequence is from Richard Heins' vector pRAH0010). Vector has C-terminal TEV cleavage site, Strept tag, 6xHIS tag.
JPUB_005028pSY066PLASMIDHis-(-NL)LipPks1+TE
JPUB_005027pSY065PLASMIDHis-LipPks1
JPUB_005026pSY044PLASMIDHis-LipPks1+TE
JPUB_004828pTrc99a-ispC-G2-ribBPLASMIDFusion of ispC to ribB using G2 linker (see ref)
JPUB_004826pTrc99a-ispC-G2-yajOPLASMIDFusion of ispC to yajO using G2 linker (see ref)
JPUB_004824pTrc99a-ribB-GP-ispCPLASMIDFusion of ribB to ispC using GP linker (see ref)
JPUB_004822pTrc99a-ribB-G2-ispCPLASMIDFusion of ribB to ispC using G2 linker (see ref)
JPUB_004820pTrc99a-yajO-PT-ispCPLASMIDFusion of yajO to ispC using PT linker (see ref)
JPUB_004818pTrc99a-yajO-GP-ispCPLASMIDFusion of yajO to ispC using GP linker (see ref)
JPUB_004816pBbA1k-AgBIS-ispA-ispDF-idiPLASMIDAgBIS, ispA, ispD, ispF, and idi on pBbA1k
JPUB_004814pBbA1k-AgBIS-ispAPLASMIDAgBIS and ispA on pBbA1k
JPUB_004812pBbA1k-AgBIS-ispDF-idiPLASMIDAgBIS, ispD, ispF, and idi on pBbA1k
JPUB_004810pTrc99A-ribB(wt)-StrepIIPLASMIDE. coli wt ribB gene with C-terminal StrepII tag, cloned into pTrc99A
JPUB_004808pTrc99A-yajOIII-StrepIIPLASMIDE. coli yajO gene, with C-terminal StrepII tag, cloned into pTrc99A
JPUB_004806pTrc99A-ribB(G108S)-StrepIIPLASMIDE. coli ribB mutant that makes DXP from Ru5P, with C-terminal StrepII tag, cloned into pTrc99A
JPUB_004804pTrc99a-ribB (wt)PLASMIDOriginal wt ribB gene from E. coli
JPUB_004802pTrc99a-ribB(T88I)PLASMIDE. coli ribB mutant that makes DXP from Ru5P, cloned into pTrc99A
JPUB_004800pTrc99a-ribB(T106I)PLASMIDE. coli ribB mutant that makes DXP from Ru5P, cloned into pTrc99A
JPUB_004798pTrc99a-ribB(S89R)PLASMIDE. coli ribB mutant that makes DXP from Ru5P, cloned into pTrc99A
JPUB_004796pTrc99a-ribB(G92D)PLASMIDE. coli ribB mutant that makes DXP from Ru5P, cloned into pTrc99A
JPUB_004794pTrc99a-ribB(G108S)PLASMIDE. coli ribB mutant that makes DXP from Ru5P, cloned into pTrc99A
JPUB_004792pTrc99A-yajOIIIPLASMIDoriginal yajO as annotated in MG1655, starting MQYN, cloned into pTrc99A
JPUB_005961pSP43PLASMIDyersiniabactin PKS with H640A mutation
JPUB_005960pSP42PLASMIDyersinaibactin PKS with H741A mutation
JPUB_004970pARH098PLASMIDE. coli codon-optimized version of the BorA1 gene from S. parvulus
JPUB_006995pA5c-MTDA-T21-MKcoPLASMIDpA5c-MTDA-T21-MKco
JPUB_006997pA5c-MTSA-T21-MKcoPLASMIDpA5c-MTSA-T21-MKco
JPUB_006999pA5c-MevT(CO)-T21-MKcoPLASMIDMevT(CO) contains codon-optimized HMGR and HMGS from S. cerevisiae. MKco = codon-optimized MK
JPUB_007001pA5c-MevT(O)-T21-MKcoPLASMIDMevT(O) = original MevT from S. cerevisiae. MKco= codon-optimized MK from S. cerevisiae. AtoB from E. coli.
JPUB_006472pARH176PLASMIDBorA2 extension module variant. Letter refers to reductive loop source (AurB, IdmO, NanA2, SpnB), number refers to N-terminal junction. xxxTE denotes TE if present, "BorDH2" denotes DH swap with BorMod2
JPUB_006470pARH164PLASMIDBorA2 extension module variant. Letter refers to reductive loop source (AurB, IdmO, NanA2, SpnB), number refers to N-terminal junction. xxxTE denotes TE if present, "BorDH2" denotes DH swap with BorMod2
JPUB_006468pARH163PLASMIDBorA2 extension module variant. Letter refers to reductive loop source (AurB, IdmO, NanA2, SpnB), number refers to N-terminal junction. xxxTE denotes TE if present, "BorDH2" denotes DH swap with BorMod2
JPUB_006466pARH162PLASMIDBorA2 extension module variant. Letter refers to reductive loop source (AurB, IdmO, NanA2, SpnB), number refers to N-terminal junction. xxxTE denotes TE if present, "BorDH2" denotes DH swap with BorMod2
JPUB_006464pARH159PLASMIDBorA2 extension module variant. Letter refers to reductive loop source (AurB, IdmO, NanA2, SpnB), number refers to N-terminal junction. xxxTE denotes TE if present, "BorDH2" denotes DH swap with BorMod2
JPUB_011106pARH167PLASMIDE. coli codon optimized BorA4ACP cloned into pET28a
JPUB_004691pDONR_At1g80290.1PLASMIDpDONR_At1g80290.1, GT64, no stop, cloned into pDONR223
JPUB_011960pET29b-plusPLASMIDDOE JGI DNA Synthesis Program standard vector. Insertion at EcoRV site.
JPUB_011962pJH10PLASMIDExpression plasmid for producing HIPP and HDMAPP in E. coli.
JPUB_011964pJH15PLASMIDThis plasmid contains FPPS1 (with his tag) and FPPS2 (with his tag) from Choristoneura fumiferana. for the production of FPP and FPP homologues in an operon with epi-Isozizaene synthase (with his tag). IPTG inducible. Inspired by JBEI-6408.
JPUB_008528pvc5020PLASMIDptsI behind lacUV5 on p15A
JPUB_008530pvc5018PLASMIDgalP-glk behind lacUV5 on p15A
JPUB_007003pTrc99a-PMDscPLASMIDPMD from S. cerevisiae cloned in pTrc99a backbone
JPUB_007005pTrc99a-PMDsc-NudBPLASMIDPMD from S. cerevisiae and NudB from E. coli
JPUB_006286B5K.MKPLASMIDexpresses fadM in one operon, and fadB + M.lut coA oxidase in a second operon. Produces methyl ketones from acyl-CoAs

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