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JBEI

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Registry Entries(12,525)

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ICE IDNameTypeDescription
JPUB_011014JBEI-13512STRAINStrain for homogenous titration of lipid unsaturation in glucose media. Arabinose concentration (1-100mM) controls proportion of unsaturated lipids. Unsaturated fatty acid auxotroph in the absence of arabinose; must be grown with oleic acid supplement.
JPUB_011012JBEI-13511STRAINStrain for homogenous titration of lipid unsaturation in non-glucose media. Arabinose concentration (0.1-10uM) controls proportion of unsaturated lipids. Unsaturated fatty acid auxotroph in the absence of arabinose; must be grown with oleic acid supplement.
JPUB_011011JBEI-056511STRAINpBAD titratable strain background BW277832T with fabB replaced in frame with CAT via lambda red. Strain is an unsaturated auxotroph; must be grown in the presence of oleic acid or other unsaturated fatty acids.
JPUB_006610JBEI-12944STRAINUDP-Xylose Transporter3 in pEarleyGate 101
JPUB_006608JBEI-12942STRAINUDP-Xylose Transporter2 in pEarleyGate 101
JPUB_006606JBEI-12939STRAINUDP-Xylose Transporter1 in pEarleyGate 101
JPUB_006604JBEI-12583STRAINUDP-Xylose Transporter3 in pENTR/SD/D no stop
JPUB_006602JBEI-12579STRAINUDP-Xylose Transporter2 in pENTR/SD/D no stop
JPUB_006600JBEI-12582STRAINUDP-Xylose Transporter1 in pENTR/SD/D no stop
JPUB_007456JBEI-12574STRAINJW5114 rcdA mutant, kanamycin resistance cassete removed by pCP20
JPUB_007454JBEI-12940STRAINsee plasmid entry
JPUB_007452JBEI-12934STRAINsee plasmid entry
JPUB_007450JBEI-12906STRAINsee plasmid entry
JPUB_007448JBEI-12905STRAINsee plasmid description
JPUB_007446JBEI-12925STRAINsee plasmid entry
JPUB_007444JBEI-12927STRAINsee plasmid entry
JPUB_007442JBEI-12935STRAINsee plasmid entry
JPUB_007441JBEI-12929STRAINFlorence Mingardon's strain. BW25113 dmsD mutant + pBbA8c-RFP
JPUB_006982JBEI-12571STRAINPlasmid containing the novel one-carbon assimilation pathway driven by pTrc promoter.
JPUB_011001JBEI-12584STRAINEHYL10 (JBx_055413), pGPDIN-fadB and pFBAIN-aco were integrated with linearized pEHYl087 (JBx_056155). The Yarrowia selection marker was excised. The E. coli fadB (EcDH1_4135) and the M. luteus aco (Mlut_11700) were codon-optimized for expression in S. cerevisiae. BsaI and BsmBI restriction sites were removed to allow for Golden Gate assembly. A three amino acid long Yarrowia peroxisome targeting signal (SKL) was added to both the fadB and aco carboxy-terminus.
JPUB_010797JBEI-13024STRAINN/a
JPUB_011000JBEI-12586STRAINEHYL01 (JBx_055370), UAS1B2-pTEF-fadM was integrated with linearized pEHYl062 (JBx_049832). The Yarrowia selection marker was excised. The E. coli fadM (EcDH1_3166) was codon-optimized for expression in Y. lipolytica. A three amino acid long Yarrowia peroxisome targeting signal (SKL) was added to the fadM carboxy-terminus.
JPUB_010999JBEI-12580STRAINEHYL01 (JBx_055370). YALI0E18876g was deleted with linearized pEHYl090 (JBx_055383). It contains the gene of ura3 flanked by loxP sites.
JPUB_010998JBEI-12573STRAINYarrowia lipolytica Po1f (JBx_055369). YALI0E18568g was deleted with linearized pEHYl018 (JBx_048825). The Yarrowia selection marker was excised.
JPUB_007421JBEI-12551STRAINE. coli DH10B
JPUB_007419JBEI-12552STRAINE. coli DH10B
JPUB_007417JBEI-12542STRAINE. coli DH10B
JPUB_007415JBEI-12545STRAINE. coli DH10B
JPUB_008929JBEI-12397STRAINDH10B harboring pS8a-PMDsc_K22M
JPUB_011897JBEI-12358STRAINpCWori-GFP in DH10b
JPUB_010796JBEI-12289STRAINprHMG1 - Hyg - Ef_MvaE
JPUB_010795JBEI-12313STRAIN2u ApLIS + LEU2 (Gal1/10)
JPUB_010794JBEI-12314STRAIN2u Le_LIS_truncAA43 + LEU2 (Gal1/10)
JPUB_010793JBEI-12334STRAIN2u Le_LIS + LEU2 (Gal1/10)
JPUB_010792JBEI-12332STRAIN2u PfLIS_truncAA61 + LEU2 (Gal1/10)
JPUB_010791JBEI-12306STRAIN2u FaLIS_truncAA19 + LEU2 (Gal1/10)
JPUB_010790JBEI-12303STRAIN2u PfLIS_truncAA34 + LEU2 (Gal1/10)
JPUB_010789JBEI-12304STRAIN2u PfLIS + LEU2 (Gal1/10)
JPUB_010788JBEI-12298STRAIN2u FaLIS + LEU2 (Gal1/10)
JPUB_010787JBEI-12299STRAIN2u ApLIS_truncAA26 + LEU2 (Gal1/10)
JPUB_010786JBEI-12279STRAINCB Linalool Synthase + Leu2
JPUB_010785JBEI-12285STRAINpGal1/10 - mvaS - pADH1 - tHMGR
JPUB_010784JBEI-12291STRAINIDI1 - Gal1/10 - EGR20mut
JPUB_00659928.1 #8 (uxt1-1)SEEDAt2g28315
JPUB_006598uxt2uxt3 (19x1 #13 #1)SEEDAt2g30460 x At1g06890 double mutant
JPUB_00659728.1SEEDAt2g28315
JPUB_00659628.1 #9SEEDAt2g28315
JPUB_00659528.1 #12SEEDAt2g28315
JPUB_00659428.2 #10 (uxt1-2)SEEDAt2g28315
JPUB_006593uxt3-1(1.1)SEEDAT1G06890

12,525 entries · Page 225 of 251